Activity was evaluated using 1% starch solution in 20 mM sodium phosphate buffer pH
6.9, containing 6.0 mM NaCl as substrate (Worthington, 1993). A total of 0.5 ml of
substrate solution was added to 0.5 ml of enzyme preparation followed by 3 min of
incubation. This was followed by addition of 0.5 ml of dinitrosalicylic acid and incubation
in boiling water bath for 5 min. Absorbance value at 540 nm was recorded. Amount of
maltose released from this assay was determined from standard curve. Specific enzyme
activity (U) was calculated a