For highly porous form such as sponges or scaffolds, the induction of theb-sheet formation of silk fibroin to make the
water-stable materials usually results in their high shrinkage leading to a difficulty in controlling shape and size of materials. Thus, the objective of this study was to improve dimensional stability of silk fibroin sponge by incorporating chitin
whiskers as nanofiller. Chitin whiskers exhibited the average length and width of 427 and 43 nm, respectively. Nanocomposite sponges at chitin whiskers to silk fibroin weight ratio (C/S ratio) of 0, 1/8, 2/8, or 4/8 were prepared by using a
freeze-drying technique. The dispersion of chitin whiskers embedded in the silk fibroin matrix was found to be homogeneous. The presence of chitin whiskers embedded into silk fibroin sponge not only improved its dimensional stability but
also enhanced its compression strength. Regardless of the chitin whisker content, SEM micrographs showed that all samples possessed an interconnected pore network with an average pore size of 150lm. To investigate the feasibility of the
nanocomposites for tissue engineering applications, L929 cells were seeded onto their surfaces, the results indicated that
silk fibroin sponges both with and without chitin whiskers were cytocompatible. Moreover, when compared to the neat
silk fibroin sponge, the incorporation of chitin whiskers into the silk fibroin matrix was found to promote cell spreading.
2007 Elsevier Ltd. All rights reserved.
For highly porous form such as sponges or scaffolds, the induction of theb-sheet formation of silk fibroin to make thewater-stable materials usually results in their high shrinkage leading to a difficulty in controlling shape and size of materials. Thus, the objective of this study was to improve dimensional stability of silk fibroin sponge by incorporating chitinwhiskers as nanofiller. Chitin whiskers exhibited the average length and width of 427 and 43 nm, respectively. Nanocomposite sponges at chitin whiskers to silk fibroin weight ratio (C/S ratio) of 0, 1/8, 2/8, or 4/8 were prepared by using afreeze-drying technique. The dispersion of chitin whiskers embedded in the silk fibroin matrix was found to be homogeneous. The presence of chitin whiskers embedded into silk fibroin sponge not only improved its dimensional stability butalso enhanced its compression strength. Regardless of the chitin whisker content, SEM micrographs showed that all samples possessed an interconnected pore network with an average pore size of 150lm. To investigate the feasibility of thenanocomposites for tissue engineering applications, L929 cells were seeded onto their surfaces, the results indicated thatsilk fibroin sponges both with and without chitin whiskers were cytocompatible. Moreover, when compared to the neatsilk fibroin sponge, the incorporation of chitin whiskers into the silk fibroin matrix was found to promote cell spreading.2007 Elsevier Ltd. All rights reserved.
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