mycelia were soaked in distilled water at a ratio of 1:20 and
double-boiled for 30 minutes, left to cool and filtered through
grade 4 Whatman filter paper. The hot aqueous extract was
then freeze-dried at −50°C ± 2°C for 48 hours and stored
at 4°C in airtight containers.
The freeze-dried hot aqueous
extract of the mycelia was used as the reducing and stabilizing
agent for the reduction of Ag+ to Ag0