To prepare the gradient mobile phases, 18 MX water, 0.250 M sodium hydroxide (Baker, Deventer, The Netherlands) and 1.0 M sodium acetate (Dionex Benelux, Amsterdam, The Netherlands) were used
To prepare the gradient mobile phases, 18 MX water, 0.250 M sodium hydroxide (Baker, Deventer, The Netherlands) and1.0 M sodium acetate (Dionex Benelux, Amsterdam, The Netherlands) were used