Ninety-six isolates could lipid production 0.1-2.8 g/l using 5G5M medium rotary shaker at 28°C until 7 day. L. starkeyi IK14ADr3 was highest lipid accumulation as 2.82 g/l while L. starkeyi 10381 was accumulated lipid as 2.86 g/l. This strain was reference strain for lipid production. Then 7 isolated which different genus and high yield lipid production was selected to study fatty acid composition and the nitrogen source of culture media for lipid accumulation.