Statistics. The concentrations indicated in the text or in the figures
represent the final organ bath concentrations of respective agonists.
Data were expressed as the mean ± the standard error of the mean
(SEM) and were analyzed using the statistical software package
GraphPad Prism 4. Two-way ANOVA with Bonferroni multiplecomparisons
post-test was used to compare concentration−response
curves obtained in aortic rings. Western blotting results among
different groups were analyzed by using one-way analysis of variance
(one-way ANOVA), and the differences in pairs of means were
determined by Newman−Keuls test. Statistical significance of the
difference was accepted at P values of