About 100 g of the powdered plant material was extracted
with 300 mL of methanol under reflux, on a water bath at 60 1C
for 1.5 h and then filtered. The extraction process was repeated
two more times, with fresh solvent each time and the combined
filtrate was concentrated under vacuum (400 mm of Hg) at 60 1C,
to obtain a dry methanolic extract.