To study the colonization pattern of inoculated bacteria, cultures
of G. diazotrophicus PAL 5 or UAP 5541/pRGS561 were centrifuged
and resuspended in the same volume of pH 6.0 phosphate saline
buffer (PBS) and used as inocula (Luna et al., 2010). For greenhouse
experiments bacterial cultures (approximately 1.109 CFU ml−1)
were used as inocula.