Entrapment efficiency
A sample of each dispersions containing 50 mg of ALA was prepared
as explained previously. In order to determine the amount of
drug that was successfully entrapped inside the cubosomes (EE%),
it was first mandated to separate the cubosomes from the resulting
dispersion. Then the amount of free drug in the dispersion was
then analyzed spectrophotometrically at kmax 250 nm, which was
then subtracted from the total amount of drug initially added