A 1g sample was weighed into a beaker and macerated
with 10ml mixture of acetone and n-hexane (1:1) and
filtered. 10ml of 50% (NH4)2SO4 solution was added,
vigorously shaken and allowed to settle. Then the upper
layer was collected and the absorbance read in Pye Unicam
double beam Spectrophotometer at 450nm against hexane
as blank [24]