Selective Isolation of Endophytic Actinomycetes: The
leaves samples were washed in running tap water to
remove soil particles. The washed leaves (1 g) were
sterilized by treating with 75% ethanol for 5 minutes then
with 1% s odium hypochlorite (NaClO) for 20 minutes.
The samples were washed in sterile distilled water three
times to remove the surface sterilization agents and
soaked in 10% sodium hydrocarbonate (NaHCO3) for 5-10
minutes to disrupt the plant tissues and inhibit fungal
growth