Pierce
the
cover
of
well(s)
of
Row B
with
the
tweezers.
Follow
same
procedure
for
remaining
rows
at
end
of
each
incubation
period.
Gently
shake
off
excess
liquid
onto
a
tissue.
Insert
Comb
in
well(s)
of
Row B
and
incubate
for
2 minutes
.
Shake
off
and
transfer
the
Comb
to
well(s)
of
Row C
and
incubate
for
5 minutes
.
Similarly,
place
the
Comb
in
well(s)
of
Row D
for
2 minutes
,
Row E
for
2 minutes
,
and
Row F
for
5 minutes
,
allowing
the
color
reaction
process
to
develop.