This DNA crosslinking by busulfan prevents DNA replication, and the cellular machinery cannot repair DNA crosslinks; thus, the cancer cells undergo apoptosis [7]. Busulfan is used for the chemo- therapeutic treatment of chronic myeloid leukemia, as it is a low- cost drug. However, following treatment, busulfan exhibits adverse effects in various organs, including the reproductive system. For example, busulfan treatment induces azoospermia and testicular atrophy in young male patients, resulting in sterility in certain cases.