Microfluidics experiment :
We did a microfluidics experiment (MITOMI : Mechanically Induced Trapping Of Molecular Interactions) in order to visualize the nanoparticles digestion by the enzyme MMP2. We had some difficulties making the chip (one button was not working), so that in the end neutravidin was on the surface everywhere, instead of being only present on distinct spots. However, we were still able to see that our biotinylated nanoparticles were binding to neutravidin (Figure 17 A).
Additionally, it seems that the addition of MMP2 (in the first four channels) results in less fluorescence.
The experiment should however be repeated, since we had many problems with the chip. Indeed, another difficulty was that the buffers (flown on the lower part) affected the chip material, so that we don't have our controls.