Our previous study indicated that persimmon tannin showed
significant inhibition in hepatic lipid accumulation in high-fat
diet fed rats (Zou et al., 2014). In this study, we evaluated the
inhibitory effect of persimmon tannin on HOA induced lipid accumulation
in L02 cells. As shown in Fig. 3, at concentrations
from 0.5 to 5 g/ml, persimmon tannin reduced the intracellularTG
content in a dose-dependent manner, but at concentration
of 10 g/ml, the inhibitory effect of persimmon tannin on TG
accumulation decreased. The inhibitory effect of persimmon
tannin onTG accumulation was not in time-dependent manner.
After incubation for 12 h, persimmon tannin displayed a
maximum TG decrease of 25.7% at concentration of 5 g/ml.
To explore the structure–activity contribution of persimmon
tannin subunits, we co-treated L02 cell lines with HOA
and four characteristic units of persimmon tannin, namely
A-type EC dimer, B-type EC dimer, A-type ECG dimer and A-type
EGCG dimer. At concentration of 40 g/ml, B-type EC dimer