A total of 183 isolates of microorganisms were collected from forest soil with the shrimp
wastes deposited. The selected four bacterial isolates with high activity of chitosanolytic enzymes on
chitosanase detected agar plates (CDA-plates) were TP11.03, TP11.22, TP12.24 and KU22.13. Among
them, the potential isolate TP12.24 was identified as Bacillus cereus which produced the highest amount
of chitosanase on M9-Chitosan medium.
Key words: chitosan, chitosanase, Bacillus cereus, isolation