Storage
Store sealed bottle containing the dehydrated medium at 2 - 30°C. Once opened and recapped, place container
in a low humidity environment at the same storage temperature. Protect from moisture and light by keeping
container tightly closed.
Expiration
Refer to expiration date stamped on the container. The dehydrated medium should be discarded if the
appearance has changed from the original color or texture. Expiry applies to medium in its intact container when
stored as directed.
Limitations of the Procedure
Due to nutritionalIn 1890, Smith used fresh unheated animal tissue for cultivating anaerobic organisms.1
Tarozzi confirmed
Smith’s findings and discovered meat broth could be heated to 104 – 105°C for 15 minutes without destroying
nutrients.2
A steam sterilized emulsion of brain tissue in water was employed by von Hibler.3,4 von Hibler found
organisms in cooked brain broth were less susceptible to harmful effects of toxic metabolic products than in
carbohydrate serum media.3,4 Robertson substituted beef heart for brain tissue and Cooked Meat Medium is
prepared according to this formula.5
Cooked Meat Medium initiates growth from a small inoculum, important for clinical specimens. Cooked Meat
Medium is recommended in standard methods for food testing.6,7 Cooked Meat Medium provides an effective
maintenance medium. This medium can be used to differentiate saccharolytic from proteolytic Clostridium spp.8
Saccharolytic species rapidly form acid and gas without digesting meat. Proteolytic species break down meat to
amino acids and form sulfur compounds (blackening and putrid smell).
Principles of the Procedure
The nitrogen, vitamin, and carbon sources are provided by Enzymatic Digest of Animal Tissue and Beef Heart.
The low concentration of Dextrose is sufficient as the energy source, but not high enough to accumulate toxic
metabolites. Sodium Chloride maintains the osmotic balance. Solid meat particles provide favorable growth
conditions for anaerobes due to reducing action of -SH (sulfhydryl) groups of muscle protein.2-4
Sulfhydryl groups
are more accessible in denatured proteins, therefore use of cooked meat particles is preferred.
Storage
Store sealed bottle containing the dehydrated medium at 2 - 30°C. Once opened and recapped, place container
in a low humidity environment at the same storage temperature. Protect from moisture and light by keeping
container tightly closed.
Expiration
Refer to expiration date stamped on the container. The dehydrated medium should be discarded if the
appearance has changed from the original color or texture. Expiry applies to medium in its intact container when
stored as directed.
Limitations of the Procedure
Due to nutritionalIn 1890, Smith used fresh unheated animal tissue for cultivating anaerobic organisms.1
Tarozzi confirmed
Smith’s findings and discovered meat broth could be heated to 104 – 105°C for 15 minutes without destroying
nutrients.2
A steam sterilized emulsion of brain tissue in water was employed by von Hibler.3,4 von Hibler found
organisms in cooked brain broth were less susceptible to harmful effects of toxic metabolic products than in
carbohydrate serum media.3,4 Robertson substituted beef heart for brain tissue and Cooked Meat Medium is
prepared according to this formula.5
Cooked Meat Medium initiates growth from a small inoculum, important for clinical specimens. Cooked Meat
Medium is recommended in standard methods for food testing.6,7 Cooked Meat Medium provides an effective
maintenance medium. This medium can be used to differentiate saccharolytic from proteolytic Clostridium spp.8
Saccharolytic species rapidly form acid and gas without digesting meat. Proteolytic species break down meat to
amino acids and form sulfur compounds (blackening and putrid smell).
Principles of the Procedure
The nitrogen, vitamin, and carbon sources are provided by Enzymatic Digest of Animal Tissue and Beef Heart.
The low concentration of Dextrose is sufficient as the energy source, but not high enough to accumulate toxic
metabolites. Sodium Chloride maintains the osmotic balance. Solid meat particles provide favorable growth
conditions for anaerobes due to reducing action of -SH (sulfhydryl) groups of muscle protein.2-4
Sulfhydryl groups
are more accessible in denatured proteins, therefore use of cooked meat particles is preferred.
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