2.6. Effect of viable bacteria on LDH-producing lactate concentration
The effect of viable LDH-producing bacteria on in vitro lactic acidosis was studied
using different strains of bacteria. One gram (wet weight) of each strain of bacterial
cells was suspended in 1.2 ml of distilled water. Then 0.3 ml of the suspended cells
was added four times to the rumen fermentation system intermittently, at an interval
of 2 h, after 12 h of incubation. For the control experiment, only distilled water was
used. Incubation was terminated after 20 h, when lactate concentrations and pH were
measured