The collected samples were washed under running water
thoroughly and then air-dried. The cleaned stems, roots and buds
were cut into pieces of about 1. 5 cm long, leaves were cut into
pieces of about 1 cm
3
size. The cut stems, roots, leaves and buds
were surface-sterilized as follows: 75% ethanol (v/v) for stems,
roots and buds 1 min, leaves 30 s and 5% sodium hypochlorite (v/v)
for stems, roots and buds 2 min, leaves 45 s, then rinsed in sterile
distilled wa te r five times. The sterilized stems, roots and buds were
opened longitudinally, leaves were cut into the shape of a comb.
The longisection of the opened stems and roots were attached to