All the studies conducted were approved by the Institutional Animal Ethical Committee (IAEC) of Bombay Veterinary College, Mumbai, India (REG No. MVC-IAEC-05112 /2012/CPCSEA) according to prescribed
guidelines of Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA), Government of India. All the animals were acclimatized for seven days before conducting the study [24].