2. Materials and methods
2.1. Preparation of green tea decoction (GTD) and black tea
decoction (BTD)
The decoctions were freshly prepared throughout the experimental
period. Fifty grams (50 g) of green or black tea leaves
(Camellia sinensis) purchased from local market (Tunis area center,
Tunisia)were soaked in hotwater and cooked in 1 L of distilledwater
for 15 min, then cooled to room temperature before distribution. All
studies were performed with same batches of tea to avoid possible
variations in the properties of the green or black tea sample.
2.2. Preparation of high-fat diet (HFD)
The HFD given to rats during the experimental period was
prepared in our laboratory as previously reported [13]. All