Biodiesel is a useful alternative energy resource and may be used as a substitute for petroleum-based diesel.The conventional method to produce biodiesel is to transesterify plant oils with methanol (1,2). However, the cost of biodiesel produced with this method is currently more expensive than that of conventional diesel due to high cost (70–85 %) of the raw material. Increasing interest is generated to explore ways to reduce the high cost of biodiesel, especially the cost of the raw materials (3). With the increasing focus on regenerative energy sources, researchers turn their attention to the cheap carbohydrate source, lignocellulosic materials