The formation of DNA ladder was once considered a hallmark of the apoptotic process. Subsequently, the universality of formation of the oligonucleosomal units during apoptosis was questioned, as it is known that after the initial cleavage of nuclear DNA into the larger fragments of 200 to 300 kb and 30 to 50 kb, there may not necessarily be any further fragmentation of the DNA to the lower molecular weight fragments.9 Currently, the absence of DNA laddering is not equated with the absence of apoptosis. 5 Therefore, the DNA laddering assay should be used to assess apoptosis only if there is similar evidence from other histochemical and cell viability assays.