In brief, standard curve using chlorogenic acidwas made.1.5mg of the extract was diluted with 1.0 mL of methanol and then mixed with 0.2 mL Folin-Ciocalteau reagent (SigmaeAldrich), 2 mL of distilled water and 1 mL of 15% Na2CO3.
In brief, standard curve using chlorogenic acidwas made.1.5mg of theextract was diluted with 1.0 mL of methanol and then mixed with0.2 mL Folin-Ciocalteau reagent (SigmaeAldrich), 2 mL of distilledwater and 1 mL of 15% Na2CO3.