2.3.5 Mechanical Properties
The Stress at break (T) and the elongation at break (E) of the biofilms were obtained using the method
D882 (ASTM, 1995a), using a texturometer TA.XT2 (Stable Microsystems, UK). The resistance to traction
was expressed as the maximum tensile strength divided by the initial cross-sectional area of the film strip
and the elongation at break, as a percentage of the original length.
2.3.6 Surface characteristic
For visualization of the surface characteristics of the films, it was used a scanning electron microscope
Shimadzu SS - 550, Superscan, software Superscan SS-550. The samples were adhered to a support
with the use of a double-sided tape of conductive carbon and subsequently metallized with gold to ensure
electrical conductivity of the surface observation.