Extraction of B vitamins was carried out according to the method outlined by Arella et al. (1996) with some modifications. Breads were divided into pieces and allowed to dry at room temperature for 2 days. Finely ground 5 g of dried bread sample was weighed in a 150 mL Erlenmeyer, 40 mL of 0.1 M HCI was added and placed in an autoclave at 121 °C for 30 min. After cooling, the pH of the mixture was adjusted to 4.5 with 2.5 M sodium acetate. The mixture was incubated in a shaking incubator (Jeio Tech, IS-971R, Seoul, Korea) at 37 °C for 48 h after the addition of 100 mg of Claradiastase with a small quantity of water. After the incubation, the extracts were filtered through a filter paper and the final extract volume was recorded. This extract was used for all B vitamin analyses with the exception of B3. B vitamins were analyzed in triplicate and the results were expressed as μg/g bread or stabilized rice bran (dry weight)