The objectives of this study was to apply molecular evidence of 16S
rDNA to construct a molecular phylogeny for ladybird beetles, examining the monophyly of
the tribes and several lower-level taxa within the group, and to establish basic phylogenetic
patterns. 16S rDNA fragments were sequenced from 47 species of in-group and two species
of out-group taxa. DNA data showed evidences of rapid evolution and saturation, having AT
and transition at a higher level. 16S rDNA gene region indicated the availability of
phylogenetic signals with the presence of skewness with significant hierarchical structure as
well as having higher number of parsimony informative characters.