AFB
1 and B(a)P were purchased from the
Sigma Chemical (St Louis, USA). These mutagens were dissolved in DMSO. The AFB1 concentration was 0.3 µg/plate for the Salmonella
tester strains TA 98 and TA 100. The B(a)P concentrations were 10 µg/plate for the Salmonella
tester strain TA 98 and 5.0 µg/plate for the Salmonella tester strain TA 100. AFB
1 and B(a)P are potent mutagens and carcinogen agents. Precautions were taken for the proper handling and
disposal of these chemicals.
Bacterial test strain