the content of a-pinene. In this work, for the first time,
changes in the membrane permeability of Escherichia coli and Enterococcus faecalis after treatment with
a-pinene or 1,8-cineole were assessed by the SYTOX Green assay. 1,8-cineole, the other main compound
present in both essential oils, was found to disrupt the cell membrane of E. coli at ½ MIC (4 mL/mL).