2.5. 8 Brix
The mean Brix of triplicate samples was measured using an Index Instrument TCR 15–30 temperature controlled refractometer accurate to 0.01 8 Brix.
2.6. Dextran breakdown products by ion chromatography with integrated pulsed amperometric detection (IC–IPAD)
See [17] for full method. Duplicate samples were diluted (1 g/25 ml) then filtered through 0.45 mm filters. All compoundsanalyzed werequantitated inreferencetostandardsin the linear range. Ethanol and mannitol were quantitated in reference to ethanol (linear range 0–1000 ppm) and mannitol (0–15 ppm), leucrose in reference to isomaltose (0–50 ppm), andisomaltotriosein reference toisomaltotriose (0–15 ppm).