Among the polymers most investigated today, there is chitosan, a polysaccharide formed by amino residues glycoside β-(1-4)-2-amino-2-deoxy-D-glucopyranose, which is obtained by deacetylation chemistry of α or β-chitin found mainly in the shells of crustaceans and mollusk’s, respectively. The great interest in producing chitosan is justified by its potential in environmental area, especially regarding the removal a high amount of azo dye from textile effluents. The chitosan was produced by Mucor subtilissimus (Mucoralean fungus) isolated from caatinga soil (PE, Brazil), employing a factorial design of 23, using Ipomea batatas L., supplemented with yeast extract and corn steep liquor (CSL) on variable response biomass and chitosan productions. The maximum 13.32g/L biomass in 96 hours (assay 3), and the higher amount of chitosan (120.96mg/g) in the central point of the factorial design were produced. The results showed that the temperature exerts a negative effect on discoloration of MB; however, the temperature at 280C removed 65% of the MB. Discoloration of MB by microbiological chitosan was more efficient at pH 6, whereas the dye molecule is a cationic character. The most promising results obtained with microbiological chitosan sorption of 92.73% of the dye MB under the condition of 8.30 mg to 20 mg of adsorbent in aqueous solution, suggesting its use in bioremediation processes. The chitosan showed higher efficiency to removal of different concentrations of dye methylene blue (MB).This microbiological polymer as an economic and eco-friendly alternative that can be applied to discoloration wastewater of dyeing industry.