of substrate in 1 min. Manganese peroxidase activity was assayed
using 50mM malonate buffer and dimethoxyphenol in 20mM
MnSO4 [24]. One unit of activity was defined as the amount of
enzyme that oxidized 1mol of dimethoxyphenol per minute and
activitieswere expressed inUl−l. Laccase activitywas assayed using
syringaldazine in 100mM sodium acetate buffer [25]. The enzymatic
reaction was carried out at room temperature and one unit
of activitywas defined as the amount ofenzymeoxidizing 1mol of
substrate in 1 min. Lignin peroxidase activitywas determined using
veratryl alcohol as a substrate [26]. One unit (U) was defined as
the amount of enzyme that oxidized 1mol of veratryl alcohol per
minute and the activity was reported as Ul−l. 1,2-Dioxygenase and
2,3-dioxygenase were measured by a modified previous method
[27]. 1,2-Dioxygenase and 2,3-dioxygenase activities were assayed
using catechol as a substrate. One unit of activitywas defined as the
amount of enzyme that oxidized 1mol of substrate per minute
and the activity was expressed in Ul−l.