The two original S. cerevisiae yeast strains were isolated from
two different regions in Catalonia, Spain. L76 from a Parellada grape
sample from “Pontons”, and L78 from a Cabernet Sauvignon grape
sample from “Conca de Barberà”. They had been selected for industrial
use on the basis of fermentation power, fermentation kinetics,
and technological properties. Yeast strains were kept
at 80 C in 20% glycerol. Pre-cultures used for the inoculation of
fermentation experiments were grown on YPD (2% glucose, 1%
yeast extract, 2% peptone) for 48 h at 28 C and 150 rpm.
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