These advantages include their biodegradability, low toxicity, better specificity for some applications, the potential for in situ production, the ability to be produced from cheap raw materials, effectiveness at extreme conditions of temperature, pH and salinity, and the organisms producing them can be modified genetically to overproduce these compounds [19, 20]. Recently biosurfactants or microorganism-produced biosurfactants have been widely used in environmental protection, including enhanced oil recovery (EOR ), oil spill control, biodegradation of distillates collected from the Czechowice-Dziedzice oil refinery are presented.