3.3. NO production
Among the most commonly employed methods to evaluate
anti-inflammatory responses is the LPS-treated Raw 264.7
murine macrophage model. Exposure of Raw 264.7 macrophages
to external bacterial toxins like lipopolysaccharide
(LPS) has been extensively shown to stimulate the secretion
of nitric oxide (NO), which is produced by the inducible isoforms
of nitric oxide synthase (iNOS) (Lee, Woo, & Kang,