Each isolate was inoculated to tomato seedlings with 5 replicates. Afterwards, pathogens were re-isolated from the discoloured stem vascular tissue and inoculated to tomatoes in order to complete Koch’s postulates. Re-isolation was done from the discoloured stem vascular tissue. Necrotic tissue fragments were
surface-sterilized (2% NaOCl) and plated on PDA.