The screening procedures for saline soils usually involve exposure to high spatial and temporal variability in field conditions
or greenhouses [16,20].
An in vitro selection procedure offers a meaningful tool for developing gene validation under controlled conditions.
A number of studies have been published on the development and isolation of salt related cell/calli lines using in vitro techniques [11,13].
In vitro culture techniques minimize environ-mental variations due to defined nutrient media, controlled conditions and homogeneity of stress applied. In addition, the