2.3. Preparation of extracts
Fifty grams of the each dry plant were homogenized using an
orbital shaker (MaxQ, Thermo Scientific, USA) at 200 rpm with
200 mL of distilled water at 60 C during 24 h then were filtered
with Whatman No.1 filter paper. The extracts were frozen with
liquid nitrogen and lyophilized, (being obtained, 1e2 g of powder)
(lyophilizer Labconco 4.5 L, Kansas, USA) for subsequent analysis.