2.2.5. Analgesic Activity
Analgesic effect of the tested extract was evaluated using the writhing method [15]. 20 mice
of both sexes weighing 25-30 gm were divided into 4 equal groups. The 1st group was kept as a
control while the 2nd was subcutaneously injected with diclofenac sodium in a dose of 5 mg/kg b.wt.
Other groups were orally given the tested extract in doses of 50 and 100 mg/kg b.wt. After 30 min,
each mouse was intraperitonealy injected with 0.25 ml of p-benzoquinone aqueous solution (0.1 mg/
ml). Thereafter, mice in all groups were observed for writhing hourly for 4 h. animals devoid of
writhing in each group were counted and the analgesic potency of the tested extract was determined as
% protection against writhing