The strain was grown in an incubator
(Percival, IA, USA) at 40 C with a 14-h light/10-h dark cycle.
Standard cyanidium medium (Toplin et al., 2008) modified to
contain twice the standard ammonium sulfate concentration and
supplemented with vitamin component of f/2 algal medium
(Andersen, 2005) was used to maintain the algae feedstock.