The content of various constituents of fermented MLBBR
beverage such as sucrose (968.28), protein (984.13), calcium and
iron (985.35) were determined using standard procedures (AOAC,
2012). The total carbohydrate (TC) content was determined by
phenol-sulphuric acid method (DuBois, Gilles, Hamilton, Rebers, &
Smith, 1956), and viable count of lactic cultures was determined by
pour plating appropriate serial dilution using MRS agar and incubated
at 37 _C for 48 h and the results were indicated as cfu/ml.