The HA/chitosan composite was prepared by the same method described above for the preparation of nano HA with some modifications. The soft nano HA precipitate obtained from the above reaction was completely dispersed in a 5 and 10 wt% chitosan (Sigma, USA) aqueous solution separately under stirring (1000 rpm) and continued the reaction for another 6 h at 37°C followed by microwave irradiation for 5–10 min. The chitosan aqueous solution was prepared by dissolving the appropriate amount of chitosan flakes into distilled water (100 ml) containing 2% of acetic acid. Finally, the composite obtained were vacuum dried and intended for further characterization.