When comparing acute alcohol administration at 0.0 g/kg and 0.5 g/kg, the trend analysis for the entire 30-min trial taking all groups into account revealed no difference. However, the trend analysis comparing only the 0.0 g/kg and 0.5 g/kg doses revealed a statistically significant time-dependent difference that was related to general activity, where an interaction between group and time was found. Animals administered the 0.5 g/kg dose had higher initial general activity that declined over time compared to the 0.0 g/kg dose, where activity increased over time. In line with this
finding, the parameter total activity was also higher, but above the level for statistical significance, during the first 15 min in rats administered alcohol at 0.5 g/kg relative to 0.0 g/kg. This finding may point toward the MCSF as a behavioral test sensitive enough to reveal acute alcohol-induced motor stimulating effects in rats, which previously have been difficult to demonstrate in outbred and/or unselected rats