while the apical side was lined with microvilli and enriched in secretion vesicles (Fig. 2A). Next, anti-GAP 65 antibodies were applied followed by gold-conjugated secondary
antibodies to delineate GAP 65 localization within the epithelial cells.
We found that GAP 65 is strongly associated with intracellular membranes,
including concentrations of gold particles in what is apparently
the Golgi complex (Fig. 2B), and in suspected secretion vesicles (Fig. 2C,
left and right images). Scanning of microvilli found at the apical secreting
edges of the epithelial cells revealed increased concentrations of
gold labeling as compared to labeling seen in adjacent inner cell regions
(Fig. 2D).