Linear calibration curves, at
six different concentrations, were defined by best-fit line equations
(Table 2). The limit of detection (LOD) and limit of quantification
(LOQ) were determined using the signal-to-noise ratio (S/N) as
shown in Table 2. Spiking of samples was used to verify the recovery of the vitamins tested (Trisconi, Campos-Gimenez,
Jaudzems, & Dowell, 2012).