pncA (left) and oxyR (right) allele-specific PCR. Amplifications were performed with chromosomal DNA from bovine M. bovis (B), goat M. bovis (G), and M. tuberculosis (T) strains. Lanes: b, reaction specific for M. bovis pncA or oxyR sequences; t, reaction specific for M. tuberculosis pncA or oxyR sequences.