The effect of substrate–extractant ratio on the yield of pectin obtained using various extractants and pHs with at 95 C for 1.5 h is shown in Table 1 . Decreasing substrate–extractant ratio from 1:25 to 1:10 (w/v) using citric acid at pH 2.5 significantly increased (p < 0.05) the yield of pectin. The highest yield of pectin was obtained using citric acid at pH 2.5 using a substrate–extractant ratio of 1:10 (w/v) [Table 1 ]. This confirms the efficiency of citric acid at pH 2.5 to extract pectin from cocoa husks. There was, however, no effect on increasing the substrate–extractant on the yield of pectin using water, citric acid at pH 4.0 or hydrochloric acid at pH 2.5 or 4.0 (Table 1 ). According to Kulkarni and Vijayanand (2010), when all the pectins were completely extracted, further increase in the volume of extractant did not show any significant increase on the pectin yield.