The cases without solution were moistened with a 1 ml drop of amoeba
Page ,s Saline buffer before centrifugation and process.
The storage cases were wiped with a sterile cotton swab
soaked with amoeba Page ,s Saline to scrape over the
internal surface of the contact lens cases then subjected to
culture. The pellet and the swab were inoculated onto nonnutrient agar plates which were seeded with non-activate
E.coli, incubated at 35.5°C for 7 days for the presence of
trophozoites and/or cysts14. A. castellanii ATCC 30234 strain
was cultured as positive control which cultured in the same
conditions. In order to isolate Acanthamoeba from contact lens cases, The Acanthamoeba cysts were examined and
groups identifid on the plate by their encysted stage size
and morphological features according to the criteria of
Pussard and Pons and Visvesvera7, 9, 15.