Figure 6. Proportions of WUS-repressed and WUS-induced genes (Busch et al., 2010) amongst differentially expressed genes (DEGs) identified in transcriptome
analysis of wus loss-of-function mutants during conversion of lateral root promordia (LRP) to shoot meristems (SMs) are consistent with rapid transcriptional
responses to WUS expression in cLRP.
(a) A comparison of a wus loss-of-function mutant (SAIL_150_G06) with the wild type (WT). WUS reporter expression was confined to scattered cells outside
cLRP after 19 h of treatment with 2iP, and ratios of WUS-induced and WUS-repressed genes amongst the DEGs suggests WUS-responsive gene expression has
not been perturbed. After 30 h of treatment with 2iP, WUS reporter expression was found within cLRP, and WUS-repressed genes now represent the majority of
DEGs with increased expression in wus mutants, and made a reduced contribution to DEGs with lower expression in the mutant. After 48 h of treatment with
2iP, no WUS-repressed genes were found amongst DEGs with lower expression in wus.
(b) In an additional comparison of two wus loss-of-function mutants with WT after 30 h of treatment with 2iP, WUS-repressed genes again outnumbered WUS-induced
genes amongst DEGs with increased expression in wus, and WUS-induced genes outnumber WUS-repressed genes amongst DEGs with lower expression in wus.