The formation of pheophytin occurs in vivo as well as in vitro. Pheophytin occurs naturally in the plant leaves and
is important as the first electron carrier intermediate in the electron transfer pathway of photosystem II in plants.
Furthermore, pheophytin has been reported to have antioxidant activity7. Here, we report our preliminary study in
isolation and identification of pheophytin from green tea leaves as well as to assay the antioxidant activity of it.